Involvement of pulmonary neuroendocrine cells (PNEC) in cystic fibrosis

Cystic fibrosis (CF) is a many visit autosomal recessive mildew which affects a physiology of a lung. It is due to mutations in a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. The CFTR protein functions as a Cl- channel.

The many usual turn is a blank phenylalanine during on all sides 508 (delF508) as well as a compared pathology is a disaster of a deteriorated protein to trade rightly to a plasma membranes. We showed which anxA5 overexpression increases delF508-CFTR aspect localization. Because anxA5 is increasing by GnRH as well as since PNEC have been neuroendocrine cells, a target is to investigate their impasse in CF.

Immunochemistry will be used to daydream a GnRH receptor in tellurian lung from normal as well as CF patients. Tissues will be dissociated as well as PNEC will be privately labelled, distant (laser micro-dissection) as well as cultured. GnRH countenance will be assessed in PNEC (RT-PCR, horse opera blotting, immunochemistry). Using aspect plasmon inflection a recover of GnRH in a enlightenment middle will be assessed. The outcome of PNEC on surrounding airway epithelial cells will be studied: 16HBE14o- (wild sort CFTR expressing cells) as well as CFBE41o- (delF508CFTR expressing cells) will be co-cultured with removed PNEC possibly in hit or distant by a permeable membrane. Co-cultured 16HBE14o- as well as CFBE41o- will be submitted to 2D-Gel electrophoresis. The differentially voiced protein will be identified by mass spectrometry. Finally, a goods of co-cultured PNEC on CFTR localization as well as duty will be assessed in a epithlial cells.

To apply: Send CV to: pascal.trouve@univ-brest.fr
Expires: Jul 16, 2010